CDKN1B Antikörper (Ser10)

Produktnummer ABIN197350

Produktdetails anti-CDKN1B Antikörper

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Bindungsspezifität: Ser10

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDKN1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody detects endogenous levels of total p27Kip1 protein.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human p27Kip1 around the phosphorylation site of serine 10 (N-G-Sp-P-S).

Anwendungsinformationen

Applikationshinweise: Western blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide as preservative and 50 % Glycerol as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu CDKN1B

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Andere Bezeichnung: CDKN1B / KIP1 (CDKN1B Produkte)

Hintergrund: P27 is a cyclin dependent kinase inhibitor (CKI) which controls G1/S phase transition in the cell cycle. p27 prevents cell cycle progression by inhibiting the activity of cyclin E-Cdk2 and cyclin D-Cdk4/6, thus preventing E2F activation and S phase progression. Low p27 levels and/or cytosolic mis-localisation have been associated with the development of lung, breast, colon, oesophagus, and prostate tumours.Synonyms: Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1

Gen-ID: 1027

NCBI Accession: NP_004055

UniProt: P46527

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagie