WB, IP, IF, IHC, ICC
Wirt: Maus
Monoclonal
G104
PE
Applikationshinweise
ELISA (0.1 μg/mL)ImmunofluorescenceWestern blotting (2g/mL)Immmunoprecipitation: use Protein A and Ab at 2 g/mg protein lysate. Immunohistochemistry on paraffin embedded sections: 5-10 μg/mL 30 min RT (Staining offormalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for10-20 min. followed by cooling at RT for 20 min. ) Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody at 4 °C for up to one month or aliquot and store at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyse the transfer of ATP to a tyrosine residue on specific cell protein targets.