Fc gamma RII (CD32) Antikörper

Produktnummer ABIN1981874

Produktdetails

Target: Fc gamma RII (CD32)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Cytometry by Time of Flight (CyTOF)

Spezifität: The mouse monoclonal antibody 3D3 recognizes an extracellular epitope of CD32, a 40 kDa polymorphic transmembrane glycoprotein serving as the low affinity receptor for aggregated IgG. This antibody recognizes CD32 isoforms on B cells of all donors, but on platelets, monocytes, and granulocytes of only some donors (131R variant, but not 131H variant).

Kreuzreaktivität (Details): Human

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: purified glycosylated recombinant human FcgammaRIIa2

Klon: 3D3

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 2-6 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze. Do not use after expiration date stamped on vial label.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen

Os, Bürgler, Ribes, Funderud, Wang, Thompson, Tjønnfjord, Bogen, Munthe: "Chronic lymphocytic leukemia cells are activated and proliferate in response to specific T helper cells." in: Cell reports, Vol. 4, Issue 3, pp. 566-77, (2013) (PubMed).

Dutertre, Bonnin-Gélizé, Pulford, Bourel, Fridman, Teillaud: "A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function." in: Journal of leukocyte biology, Vol. 84, Issue 6, pp. 1511-20, (2008) (PubMed).

Boruchov, Heller, Veri, Bonvini, Ravetch, Young: "Activating and inhibitory IgG Fc receptors on human DCs mediate opposing functions." in: The Journal of clinical investigation, Vol. 115, Issue 10, pp. 2914-23, (2005) (PubMed).

Vely, Gruel, Moncuit, Cochet, Rouard, Dare, Galon, Sautes, Fridman, Teillaud: "A new set of monoclonal antibodies against human Fc gamma RII (CD32) and Fc gamma RIII (CD16): characterization and use in various assays." in: Hybridoma, Vol. 16, Issue 6, pp. 519-28, (1998) (PubMed).

Antigendetails

Target: Fc gamma RII (CD32)

Andere Bezeichnung: CD32 (CD32 Produkte)

Synonyme: CD32 antikoerper, CD32B antikoerper, FCG2 antikoerper, FCGR2 antikoerper, IGFR2 antikoerper, FCGR2B antikoerper, AI528646 antikoerper, F630109E10Rik antikoerper, Fc[g]RII antikoerper, FcgRII antikoerper, Fcgr2 antikoerper, Fcgr2a antikoerper, Fcr-2 antikoerper, Fcr-3 antikoerper, Ly-17 antikoerper, Ly-m20 antikoerper, LyM-1 antikoerper, Lym-1 antikoerper, FCRII antikoerper, FcRII antikoerper, Fc fragment of IgG receptor IIb antikoerper, Fc receptor, IgG, low affinity IIb antikoerper, FCGR2B antikoerper, Fcgr2b antikoerper

Hintergrund: Fc fragment of IgG receptor IIa,CD32 (FcgammaRII) is a low affinity receptor for aggregated IgG. It is strongly expressed on monocytes, granulocytes, myeloid and myeloblastic cell lines, and weakly on B cells, CD34+ bone marrow cells, and resting and activated platelets. After binding its ligand, CD32 induces IgG-mediated phagocytosis and oxidative burst in monocytes and neutrophils, whereas in B cells it mediates a negative signal. This polymorphic transmembrane glycoprotein is expressed not only in the activating (CD32a) and inhibitory isoform (CD32b), but also in individual variants with differing avidities for IgG subtypes (e.g. the CD32a131R and CD32a131H allotypes).,FCG2, FCGR2A, IGFR2

Gen-ID: 2212

UniProt: P12318