VASP Antikörper (pSer239)

Produktnummer ABIN198238

Produktdetails anti-VASP Antikörper

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Bindungsspezifität: AA 236-242, pSer239

Reaktivität: Human, Maus, Ratte, Affe, Hund, Rind (Kuh), Pferd

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VASP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Spezifität: Recognizes human phosphorylated VASP, Mr 50kD. Species cross-reactivity: mouse.

Aufreinigung: Gel filtration

Immunogen: KLH-conjugated, synthetic phosphopeptide corresponding to amino acids 236-242 of human phospho-VASP (RKV[pS]KQE).

Type of Immunogen: Synthetic phospho-peptide

Klon: 16C2

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.05 μg/mL), Flo (2.4 μg/mL), ICC, IP, WB

Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry, FACS and Immunoprecipitation. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: An independent laboratory has reported that 3 μg/mL antibody showed positive immunostaining in SNP or 8pCPT-cGMP-treated human platelets, fixed with 4 % paraformaldehyde and permeabilized with 0.2 % Triton-X. FACS: 2.4 μg/mL using fixed and permeabilized human platelets, as reported by an independent laboratory. Immunoprecipitation: Immunoprecipitates phosphorylated VASP from vanadate-treated A431 cells, as reported by an independent laboratory. ELISA: 0.05 μg/mL, as reported by an independent laboratory.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 1 mL ddH2O

Konzentration: Lot specific

Buffer: Supplied as a lyophilized powder from PBS, 0.09 % sodium azide, PEG, sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu VASP

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Andere Bezeichnung: VASP (VASP Produkte)

Hintergrund: Name/Gene ID: VASP
Family: Ena-VASP

Synonyms: VASP

Gen-ID: 7408

UniProt: P50552

Pathways: T-Zell Rezeptor Signalweg, Regulation of Actin Filament Polymerization, Tube Formation