GAPDH Antikörper

Produktnummer ABIN2017607

Produktdetails anti-GAPDH Antikörper

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reaktivität: Human, Schwein, Rind (Kuh), Ziege, Fisch

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GAPDH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: GAPDH Antibody, mAb, Mouse detects endogenous levels of human, pig, cow, goat, and fish GAPDH.

Kreuzreaktivität (Details): This product has been not tested with other species yet.

Produktmerkmale: Fusion Partner: Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.

Aufreinigung: Protein A affinity column

Immunogen: Human GAPDH protein from erythrocytes

Klon: 3B1E9

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 μg/mL
Western blot: 0.1-1.0 μg/mL.
Other Application: User optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.

Konzentration: 0.5 mg/mL

Buffer: Lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

Referenzen für anti-GAPDH Antikörper (ABIN2017607)

Chen, Caporizzo, Bedi, Vite, Bogush, Robison, Heffler, Salomon, Kelly, Babu, Morley, Margulies, Prosser: "Suppression of detyrosinated microtubules improves cardiomyocyte function in human heart failure." in: Nature medicine, Vol. 24, Issue 8, pp. 1225-1233, (2019) (PubMed).

Ju, Gao, Hou, Wang, Zhang, Sun, Zhang, Xu, Shi, Hu, Zhang: "Neuroprotective effect of chondroitin sulfate on SH‑SY5Y cells overexpressing wild‑type or A53T mutant α‑synuclein." in: Molecular medicine reports, Vol. 16, Issue 6, pp. 8721-8728, (2018) (PubMed).

Wang, Mir: "Andes virus nucleocapsid protein interrupts protein kinase R dimerization to counteract host interference in viral protein synthesis." in: Journal of virology, Vol. 89, Issue 3, pp. 1628-39, (2015) (PubMed).

Yang, Li, Liu, He, Yuan, Li, Cao, Kim: "Mx1-cre mediated Rgs12 conditional knockout mice exhibit increased bone mass phenotype." in: Genesis (New York, N.Y. : 2000), Vol. 51, Issue 3, pp. 201-9, (2013) (PubMed).

Cassel, Reitz: "Ubiquilin-2 (UBQLN2) binds with high affinity to the C-terminal region of TDP-43 and modulates TDP-43 levels in H4 cells: characterization of inhibition by nucleic acids and 4-aminoquinolines." in: Biochimica et biophysica acta, Vol. 1834, Issue 6, pp. 964-71, (2013) (PubMed).

Martins, Collado-Romero, Martínez-Gomáriz, Carvajal, Gil, Lucena, Moreno, Garrido: "Proteomic analysis of porcine mesenteric lymph-nodes after Salmonella typhimurium infection." in: Journal of proteomics, Vol. 75, Issue 14, pp. 4457-70, (2012) (PubMed).

Cassel, McDonnell, Velvadapu, Andrianov, Reitz: "Characterization of a series of 4-aminoquinolines that stimulate caspase-7 mediated cleavage of TDP-43 and inhibit its function." in: Biochimie, Vol. 94, Issue 9, pp. 1974-81, (2012) (PubMed).

Details zu GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Andere Bezeichnung: GAPDH (GAPDH Produkte)

Synonyme: G3PD antikoerper, GAPD antikoerper, Gapd antikoerper, BEST:GH12586 antikoerper, CG12055 antikoerper, Dmel\\CG12055 antikoerper, GA3PDH antikoerper, GADPH antikoerper, GAP antikoerper, GAPDH antikoerper, GAPDH I antikoerper, GAPDH-1 antikoerper, GAPDH1 antikoerper, GAPDHI antikoerper, Gapdh antikoerper, Gapdh-1 antikoerper, Gapdh43E antikoerper, gadph antikoerper, gapdh antikoerper, gapdh-1 antikoerper, gh12586 antikoerper, cb609 antikoerper, gapd antikoerper, mg:bb02e05 antikoerper, wu:fb33a10 antikoerper, wu:ft80f05 antikoerper, KNC-NDS6 antikoerper, g3pd antikoerper, G3PDH antikoerper, glyceraldehyde-3-phosphate dehydrogenase antikoerper, Glyceraldehyde 3 phosphate dehydrogenase 1 antikoerper, glyceraldehyde-3-phosphate dehydrogenase S homeolog antikoerper, glyceraldehyde-3-phosphate dehydrogenase, type I antikoerper, olfactory receptor 8K3 antikoerper, GAPDH antikoerper, Gapdh antikoerper, Gapdh1 antikoerper, gapdh antikoerper, gapdh.S antikoerper, gapDH antikoerper, LOC100404960 antikoerper, LOC614985 antikoerper

Hintergrund: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimers and Huntingtons disease. GAPDH Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with human GAPDH protein from erythrocytes.

Molekulargewicht: Predicted Band Size: 36 KD
Observed Band Size: 36 KD