STAT5B Antikörper (AA 774-787)
-
- Target Alle STAT5B Antikörper anzeigen
- STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))
-
Bindungsspezifität
- AA 774-787
-
Reaktivität
- Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Schwein, Hamster
-
Wirt
- Kaninchen
-
Klonalität
- Polyklonal
-
Konjugat
- Dieser STAT5B Antikörper ist unkonjugiert
-
Applikation
- Western Blotting (WB), Immunoprecipitation (IP)
- Spezifität
- Recognizes the 90-92kD forms of STAT5B protein. Species cross-reactivity: Human and mouse.
- Homologie
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig (100%) Panda, Bat, Chicken, Xenopus (93%) Opossum (86%).
- Aufreinigung
- Protein A purified
- Immunogen
- Amino acids 774-787 of human STAT5B (GRPMDSQWIPHAQS). The peptide was coupled to KLH prior to immunization. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig (100%), Panda, Bat, Chicken, Xenopus (93%), Opossum (86%).
- Isotyp
- IgG
- Top Product
- Discover our top product STAT5B Primärantikörper
-
-
- Applikationshinweise
-
Approved: IP, WB
Usage: Western Blot: 0.5-2 mg/mL detects STAT5B (doublet of 90kD and 92kD) in. human A431 and mouse 3T3 fibroblasts using enhanced chemiluminescence with a five minute exposure. Immunoprecipitation: 4 μg immunoprecipitates STAT5B from A431 carcinoma cells and a. previous lot immunoprecipitated STAT5B from human Raji cells. A RIPA lysate of human A431 cells (500 μg protein) was incubated with 4 μg of anti-STAT 5B Immune complexes were precipitated with protein A agarose and analyzed by immunoblotting with anti- STAT 5B. Bands were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP in conjunction with enhanced chemiluminescence detection. Positive Antigen Control: mouse 3T3 cell lysate. Add 2.5 μL of 2-mercaptoethanol. per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. - Kommentare
-
Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- Supplied as a liquid in 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
-
Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
-
- Target
- STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))
- Andere Bezeichnung
- STAT5B / STAT (STAT5B Produkte)
- Synonyme
- STAT5 antikoerper, stat5 antikoerper, STAT5A antikoerper, STAT5B antikoerper, signal transducer and activator of transcription 5B antikoerper, signal transducer and activator of transcription 5B S homeolog antikoerper, Signal transducer and activator of transcription 5B antikoerper, STAT5B antikoerper, Stat5b antikoerper, stat5b.S antikoerper, LOC490969 antikoerper, sta5b antikoerper, LOC100566752 antikoerper, LOC100052954 antikoerper
- Hintergrund
-
Name/Gene ID: STAT5B
Synonyms: STAT5B, Transcription factor STAT5B, STAT5 - Gen-ID
- 6777
- UniProt
- P51692
- Pathways
- JAK-STAT Signalweg, RTK Signalweg, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation
-