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CD45RA Antikörper (Biotin)

CD45RA Reaktivität: Human FACS, IF, IHC (p), WB Wirt: Maus Monoclonal 4AHI100 Biotin
Produktnummer ABIN2144324
  • Target Alle CD45RA Antikörper anzeigen
    CD45RA
    Reaktivität
    • 111
    • 9
    • 8
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    Human
    Wirt
    • 130
    • 1
    • 1
    Maus
    Klonalität
    • 131
    • 1
    Monoklonal
    Konjugat
    • 34
    • 18
    • 17
    • 9
    • 7
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD45RA Antikörper ist konjugiert mit Biotin
    Applikation
    • 124
    • 31
    • 29
    • 28
    • 20
    • 19
    • 17
    • 15
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Produktmerkmale
    4AHI100 reacts with the 220 kDa isoform A of CD45.
    Klon
    4AHI100
    Isotyp
    IgG2b
    Top Product
    Discover our top product CD45RA Primärantikörper
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Testdurchführung
    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature.
    • Repeat step 5.
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
  • Target
    CD45RA
    Andere Bezeichnung
    CD45RA (CD45RA Produkte)
    Synonyme
    B220 antikoerper, CD45 antikoerper, CD45R antikoerper, GP180 antikoerper, L-CA antikoerper, LCA antikoerper, LY5 antikoerper, T200 antikoerper, Lca antikoerper, RT7 antikoerper, protein tyrosine phosphatase, receptor type C antikoerper, protein tyrosine phosphatase, receptor type, C antikoerper, PTPRC antikoerper, Ptprc antikoerper
    Hintergrund
    This is clustered as CD45RA, and is expressed on naive/resting T cells and on medullart thymocytes. In comparison, CD45RO is expressed on memory/activated T cells and cortical thymocytes. CD45RA and CD45RO are useful for discriminating between naive and memory T cells in the study of the immune system.
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