C1q Antikörper

Produktnummer ABIN2191787

Produktdetails anti-C1q Antikörper

Target: C1q (Complement Fragment C1q (C1q))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser C1q Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoassay (IA)

Kreuzreaktivität (Details): Cross reactivity: : Yes

Sterilität: 0.2 μm filtered

Klon: 7H8

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: For immunohistochemistry, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive Macrophages, follicular dendritic cells control 1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Referenzen für anti-C1q Antikörper (ABIN2191787)

Ankeny, Guan, Popovich: "B cells produce pathogenic antibodies and impair recovery after spinal cord injury in mice." in: The Journal of clinical investigation, Vol. 119, Issue 10, pp. 2990-9, (2009) (PubMed).

Abbitt, Cotter, Ridger, Crossman, Hellewell, Norman: "Antibody ligation of murine Ly-6G induces neutropenia, blood flow cessation, and death via complement-dependent and independent mechanisms." in: Journal of leukocyte biology, Vol. 85, Issue 1, pp. 55-63, (2008) (PubMed).

Kang, Do, Lee, Park, Cheong, Lynch, Loeffler, Steinman, Park: "A dominant complement fixation pathway for pneumococcal polysaccharides initiated by SIGN-R1 interacting with C1q." in: Cell, Vol. 125, Issue 1, pp. 47-58, (2006) (PubMed).

Suresh, Singh, Ferguson, Agrawal: "Role of the property of C-reactive protein to activate the classical pathway of complement in protecting mice from pneumococcal infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 7, pp. 4369-74, (2006) (PubMed).

Zachrau, Finke, Kropf, Gosink, Kirchner, Goerg: "Antigen localization within the splenic marginal zone restores humoral immune response and IgG class switch in complement C4-deficient mice." in: International immunology, Vol. 16, Issue 12, pp. 1685-90, (2004) (PubMed).

Details zu C1q

Target: C1q (Complement Fragment C1q (C1q))

Andere Bezeichnung: c1q (C1q Produkte)

Synonyme: AI255395 antikoerper, C1q antikoerper, complement component 1, q subcomponent, alpha polypeptide antikoerper, C1qa antikoerper

Hintergrund: The monoclonal antibody 7H8 recognizes mouse CIq. Clq, a member of the 'defense collagen' family, is the first subcomponent of the Cl complex of the classical pathway of complement activation. Several functions have been assigned to the pattern recognition molecule Clq, which include antibody-dependent and independent immune functions like triggering of rapid enhanced phagocytosis resulting in efficient containment of pathogens or clearance of cellular debris, apoptotic cells and immune complexes , and is considered to be mediated by Clq receptors present on the effector cell surface. There remains some uncertainty about the identities of the receptors that mediate Clq functions. Some of the previously described Clq receptor molecules, such as gClqR and cClqR, now appear to have less of a role in Clq functions than in functions unrelated to Clq. Experiments with gene targeted homozygous Clq-deficient mice have suggested a role for Clq in modulation of the humoral immune response, and also in protection against development of autoimmunity. The first component of complement Cl is a complex of three glycoproteins - Clq, Clr, and Cls. Cls and Clr interact to form a Cazf-dependent tetrameric proenzyme complex, C lr,-C 1 s2, which makes contacts with the Clq collagen domain. Binding of Clq to immune complexes (IgG or IgM) via the gClq domain, is considered to induce a conformational change in the collagen region of Clq, which leads to the autoactivation of Clr which, in turn, activates Cls. The activated Cl complex then cleaves components C4 and C2 in the classical complement cascade.