C4
Reaktivität: Human
IEP
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive T-cell regions of mouse spleen control
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Murata, Fox-Talbot, Qian, Takahashi, Stahl, Baldwin, Wasowska: "Synergistic deposition of C4d by complement-activating and non-activating antibodies in cardiac transplants." in: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, Vol. 7, Issue 11, pp. 2605-14, (2007) (PubMed).
Paul, Pozdnyakova, Mitchell, Carroll: "Anti-DNA autoreactivity in C4-deficient mice." in: European journal of immunology, Vol. 32, Issue 9, pp. 2672-9, (2002) (PubMed).
The monoclonal antibody 16D2 recognizes mouse complement factor C4, formerly known as Gg protein, which consists of an alpha-, beta-, and gamma-chain. The classical pathway of complement and the Mannose binding lectin activation pathway converge at C4. C1s, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. Subsequently, C4b can be cleaved to C4c and C4d by other serum enzymes. The monoclonal antibody 16D2 reacts with intact C4, C4b and C4d. C4 is an acute phase protein that is produced by hepatocytes, monocytes and intestinal epithelial cells and can be used in experimental animals as a marker for activation of the classical complement pathway. Recent studies have demonstrated an association between graft rejection and C4d deposition in a mouse model for cardiac transplantation. Aliases Complement component 4 Immunogen Thymocytes decorated with Thy-1 antibody and complement components