Complement C4 Antikörper

Produktnummer ABIN2191789

Produktdetails anti-Complement C4 Antikörper

Target: Complement C4 (C4) (Complement 4 (C4))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Complement C4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität (Details): Cross reactivity: C4b : Yes, C4d : Yes

Sterilität: 0.2 μm filtered

Klon: 16D2

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive T-cell regions of mouse spleen control

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Referenzen für anti-Complement C4 Antikörper (ABIN2191789)

Murata, Fox-Talbot, Qian, Takahashi, Stahl, Baldwin, Wasowska: "Synergistic deposition of C4d by complement-activating and non-activating antibodies in cardiac transplants." in: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, Vol. 7, Issue 11, pp. 2605-14, (2007) (PubMed).

Paul, Pozdnyakova, Mitchell, Carroll: "Anti-DNA autoreactivity in C4-deficient mice." in: European journal of immunology, Vol. 32, Issue 9, pp. 2672-9, (2002) (PubMed).

Details zu Complement C4

Target: Complement C4 (C4) (Complement 4 (C4))

Andere Bezeichnung: c4 (C4 Produkte)

Hintergrund: The monoclonal antibody 16D2 recognizes mouse complement factor C4, formerly known as Gg protein, which consists of an alpha-, beta-, and gamma-chain. The classical pathway of complement and the Mannose binding lectin activation pathway converge at C4. C1s, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. Subsequently, C4b can be cleaved to C4c and C4d by other serum enzymes. The monoclonal antibody 16D2 reacts with intact C4, C4b and C4d. C4 is an acute phase protein that is produced by hepatocytes, monocytes and intestinal epithelial cells and can be used in experimental animals as a marker for activation of the classical complement pathway. Recent studies have demonstrated an association between graft rejection and C4d deposition in a mouse model for cardiac transplantation. Aliases Complement component 4 Immunogen Thymocytes decorated with Thy-1 antibody and complement components

Pathways: Komplementsystem