Siglec H Antikörper

Produktnummer ABIN2191824

Produktdetails anti-Siglec H Antikörper

Target: Siglec H

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Siglec H Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Inhibition Assay (InhA)

Sterilität: 0.2 μm filtered

Klon: 440c

Anwendungsinformationen

Applikationshinweise: For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For inhibition of IFN-alpha secretion dilutions have to be made according to the amounts of Siglec-H to be inactivated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, containing 0.1 % bovine serum albumin.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Haltbarkeit: 12 months

Referenzen für anti-Siglec H Antikörper (ABIN2191824)

Blasius, Vermi, Krug, Facchetti, Cella, Colonna: "A cell-surface molecule selectively expressed on murine natural interferon-producing cells that blocks secretion of interferon-alpha." in: Blood, Vol. 103, Issue 11, pp. 4201-6, (2004) (PubMed).

Details zu Siglec H

Target: Siglec H

Andere Bezeichnung: Siglec-H (Siglec H Produkte)

Synonyme: 6430529G09Rik antikoerper, Siglec-H antikoerper, sialic acid binding Ig-like lectin H antikoerper, Siglech antikoerper

Hintergrund: The monoclonal antibody 440c reacts with Siglec-H, a cell-surface receptor molecule selectively expressed on murine natural interferon producing cells (IPC), also called mouse plasmacytoid dendritic cells (pDC). Siglec H is unique among Sialic acid-binding Ig-like lectins (Siglecs) proteins because it associates with the adaptor protein DAP12. DAP12 recognize certain viruses and CpG-DNA through TLR9, resulting in secretion of IFN-alpha, IL-12 and proinflammatory chemokines. Together these cytokines and chemokines recruite and activate NK cells and T cells as well as modulating the antigen presenting function of dendritic cells (DC). IPC themselves also function as antigen presenting cells that expand memory T cells and induce Th1 differentiation. Therefore IPC may provide a first line of host defense against viral infections by activating both innate and adaptive responses in vivo. The monoclonal antibody 440c exclusively recognizes mouse Siglec-H in all lymphoid organs under both normal and inflammatory conditions. Siglec-H is normal present in the T cell zone of lymph nodes and spleen. Incubation of IPC with antibody 440c in vitro or administration of antibody 440c in vivo reduces secretion of IFN-alpha in response to CpG DNA without causing IPC depletion.