Ly6C Antikörper

Produktnummer ABIN2191831

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch Ly6C in FACS, IHC (fro), IHC (p), D und IA. Er zeigt eine Reaktivität gegenüber Maus und wurde in 4+ Publikationen erwähnt.

Kurzübersicht für Ly6C Antikörper (ABIN2191831)

Target: Ly6C (Ly-6C) (Ly-6C)

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Ly6C Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Detection (D), Immunoassay (IA)

Klon: ER-MP20

Produktdetails anti-Ly6C Antikörper

Sterilität: 0.2 μm filtered

Anwendungsinformationen

Applikationshinweise: For immunohistology and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Haltbarkeit: 12 months

Referenzen für anti-Ly6C Antikörper (ABIN2191831)

Manitz, Horst, Seeliger, Strey, Skryabin, Gunzer, Frings, Schönlau, Roth, Sorg, Nacken: "Loss of S100A9 (MRP14) results in reduced interleukin-8-induced CD11b surface expression, a polarized microfilament system, and diminished responsiveness to chemoattractants in vitro." in: Molecular and cellular biology, Vol. 23, Issue 3, pp. 1034-43, (2003) (PubMed).

Henkel, McKercher, Leenen, Maki: "Commitment to the monocytic lineage occurs in the absence of the transcription factor PU.1." in: Blood, Vol. 93, Issue 9, pp. 2849-58, (1999) (PubMed).

Chan, Leenen, Bertoncello, Nishikawa, Hamilton: "Macrophage lineage cells in inflammation: characterization by colony-stimulating factor-1 (CSF-1) receptor (c-Fms), ER-MP58, and ER-MP20 (Ly-6C) expression." in: Blood, Vol. 92, Issue 4, pp. 1423-31, (1998) (PubMed).

de Bruijn, Slieker, van der Loo, Voerman, van Ewijk, Leenen: "Distinct mouse bone marrow macrophage precursors identified by differential expression of ER-MP12 and ER-MP20 antigens." in: European journal of immunology, Vol. 24, Issue 10, pp. 2279-84, (1994) (PubMed).

Details zu Ly6C

Target: Ly6C (Ly-6C) (Ly-6C)

Hintergrund: The monoclonal antibody ER-MP20 specifically reacts with mouse macrophage precursor cells in the mid-stage of their development (late CFU-M, monoblasts and monocytes). The antigen is a 14 kD surface protein which is very similar to Ly-6C and may be analogous to human CD59. It is inducible by IFN-alpha, IFN-beta and IFN-gamma. In tissue sections, the antigen is found on macrophage precursor subpopulations. In the bone marrow and hemopoietic islands of the lymphoid organs, and in the spleen. Activated macrophages in inflammatory tissues also express the ER-MP20-related antigen. The monoclonal antibody ER-MP20 has been raised after immunization of rats with mouse macrophage cell lines and reacts with mouse macrophage precursor cells. The monoclonal antibody also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan- macrophage marker BM8 (anti-F4/80) is recommended. In combination with an anti-mouse CD31/PECAM-1 antibody, ER-MP20 can be used to evaluate the cellular composition in murine bone marrow (e.g. using flow cytometric analysis). ER-MP20 also detects a wide range of endothelial cells.