PI3 Antikörper

Produktnummer ABIN2191925

Produktdetails anti-PI3 Antikörper

Target: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PI3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoassay (IA)

Sterilität: 0.2 μm filtered

Klon: TRAB2O

Anwendungsinformationen

Applikationshinweise: For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For Western blotting it is recommended to use non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Haltbarkeit: 12 months

Referenzen für anti-PI3 Antikörper (ABIN2191925)

Huh, Oono, Shirafuji, Akiyama, Arata, Sakaguchi, Huh, Iwatsuki: "Dynamic alteration of human beta-defensin 2 localization from cytoplasm to intercellular space in psoriatic skin." in: Journal of molecular medicine (Berlin, Germany), Vol. 80, Issue 10, pp. 678-84, (2002) (PubMed).

Vandermeeren, Daneels, Bergers, van Vlijmen-Willems, Pol, Geysen, Schalkwijk: "Development and application of monoclonal antibodies against SKALP/elafin and other trappin family members." in: Archives of dermatological research, Vol. 293, Issue 7, pp. 343-9, (2001) (PubMed).

Schalkwijk, Wiedow, Hirose: "The trappin gene family: proteins defined by an N-terminal transglutaminase substrate domain and a C-terminal four-disulphide core." in: The Biochemical journal, Vol. 340 ( Pt 3), pp. 569-77, (1999) (PubMed).

van Bergen, Andriessen, Spruijt, van de Kerkhof, Schalkwijk: "Expression of SKALP/elafin during wound healing in human skin." in: Archives of dermatological research, Vol. 288, Issue 8, pp. 458-62, (1997) (PubMed).

Pfundt, van Ruissen, van Vlijmen-Willems, Alkemade, Zeeuwen, Jap, Dijkman, Fransen, Croes, van Erp, Schalkwijk: "Constitutive and inducible expression of SKALP/elafin provides anti-elastase defense in human epithelia." in: The Journal of clinical investigation, Vol. 98, Issue 6, pp. 1389-99, (1996) (PubMed).

Alkemade, van Vlijmen-Willems, van Haelst, van de Kerkhof, Schalkwijk: "Demonstration of skin-derived antileukoproteinase (SKALP) and its target enzyme human leukocyte elastase in squamous cell carcinoma." in: The Journal of pathology, Vol. 174, Issue 2, pp. 121-9, (1994) (PubMed).

Details zu PI3

Target: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Andere Bezeichnung: Elafin/skalp (PI3 Produkte)

Synonyme: ESI antikoerper, SKALP antikoerper, WAP3 antikoerper, WFDC14 antikoerper, cementoin antikoerper, trappin-2 antikoerper, TRAPPIN-2 antikoerper, elafin antikoerper, peptidase inhibitor 3 antikoerper, peptidase inhibitor 3, skin-derived (SKALP) antikoerper, PI3 antikoerper

Hintergrund: Elafin is an epithelial proteinase inhibitor also known under various other names such as Skin-derived Anti leukoproteinase (SKALP) and Elastase-Specific Inhibitor (ESI). Elafin belongs to the Trappin gene family and was given the systematic name Trappin-2. The Trappin family is defined by a N-terminal transglutaminase substrate domain and a C-terminal four disulphide core. Trappins have been suggested to play a role in the regulation of inflammation and in protection against tissue damage in stratified epithelia. Elafin is an inhibitor of leukocyte elastase and proteinase-3 and is a substrate for transglutaminases. The protein is constitutively expressed in various epithelia including hair follicles, oesophagus, vagina and oral cavity. Elafin is not present in normal human skin but is strongly induced during inflammation as in psoriasis and wound healing. Antibodies to Elafin can be used to evaluate the effects of treatment of psoriasis since its expression is significantly correlated with clinical scores. Antibodies to Elafin have also been succesfully used to study differentiation in squamous cell carcinoma of the head-and-neck region, oesophagus and skin. It is also shown that Elafin possesses antimicrobial activity against gram-positive and gram-negative bacteria. TRAB2O recognises all known Trappins via the GQDPVK epitope. TRAB2O shows strong reaction with human native full-length Elafin/SKALP and a weaker reaction with fully processed Elafin/SKALP (C-terminal 57 aa). For detection of fully processed Elafin/SKALP (C-terminal 57 aa) we advise to use HM2063. The antibody is cross reactive with Trappin family members of other species with GQDPVK epitope.