PRTN3 Antikörper

Produktnummer ABIN2192030

Produktdetails anti-PRTN3 Antikörper

Target: PRTN3 (Proteinase 3 (PRTN3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PRTN3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoassay (IA), Inhibition Assay (InhA), Activation (Act)

Sterilität: 0.2 μm filtered

Klon: WGM2

Anwendungsinformationen

Applikationshinweise: For flow cytometry, Western blotting and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For inhibition of biological activity of PR3 dilutions have to be made according to the amounts of proteinase 3 to be inactivated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, containing 0.1 % bovine serum albumin.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Haltbarkeit: 12 months

Referenzen für anti-PRTN3 Antikörper (ABIN2192030)

Pederzoli, Kantari, Gausson, Moriceau, Witko-Sarsat: "Proteinase-3 induces procaspase-3 activation in the absence of apoptosis: potential role of this compartmentalized activation of membrane-associated procaspase-3 in neutrophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 10, pp. 6381-90, (2005) (PubMed).

Van Der Geld, Limburg, Kallenberg: "Characterization of monoclonal antibodies to proteinase 3 (PR3) as candidate tools for epitope mapping of human anti-PR3 autoantibodies." in: Clinical and experimental immunology, Vol. 118, Issue 3, pp. 487-96, (2000) (PubMed).

Grimminger, Hattar, Papavassilis, Temmesfeld, Csernok, Gross, Seeger, Sibelius: "Neutrophil activation by anti-proteinase 3 antibodies in Wegener's granulomatosis: role of exogenous arachidonic acid and leukotriene B4 generation." in: The Journal of experimental medicine, Vol. 184, Issue 4, pp. 1567-72, (1996) (PubMed).

Braun, Csernok, Rögener-Schwarz, Ludwig, Müller-Hermelink, Gross, Feller: "Monoclonal antibody WGM1 directed against proteinase 3: an immunohistochemical marker for naphthol ASD chloroacetate." in: Hematological oncology, Vol. 14, Issue 2, pp. 83-90, (1996) (PubMed).

Csernok, Lüdemann, Gross, Bainton: "Ultrastructural localization of proteinase 3, the target antigen of anti-cytoplasmic antibodies circulating in Wegener's granulomatosis." in: The American journal of pathology, Vol. 137, Issue 5, pp. 1113-20, (1990) (PubMed).

Details zu PRTN3

Target: PRTN3 (Proteinase 3 (PRTN3))

Andere Bezeichnung: Proteinase 3 (PRTN3 Produkte)

Synonyme: ela2 antikoerper, MGC132250 antikoerper, ACPA antikoerper, AGP7 antikoerper, C-ANCA antikoerper, CANCA antikoerper, MBN antikoerper, MBT antikoerper, NP-4 antikoerper, NP4 antikoerper, P29 antikoerper, PR-3 antikoerper, PR3 antikoerper, mPR3 antikoerper, proteinase 3 L homeolog antikoerper, proteinase 3 antikoerper, prtn3.L antikoerper, PRTN3 antikoerper, Prtn3 antikoerper

Hintergrund: Monoclonal antibody WGM2 reacts with human proteinase 3 (PR3), a 30 kDa protein. PR3 is a major antigen recognized by autoantibodies directed against cytoplasmic proteins of neutrophilic granulocytes and monocytes (so called anti-neutrophil cytoplasmic autoantibodies (ANCA)). ANCA are able to activate primed neutrophils to produce oxygen radicals and release lytic enzymes, including PR3. Proteinase 3 (PR3) was identified as the target antigen of ANCA in Wegener's granulomatosis (WG). ANCA directed against PR3 (PR3-ANCA) can interfere with the binding of PR3 to its physiological inhibitor alpha1-antitrypsin (alpha1-AT) and with the proteolytic activity of PR3. At the site of inflammation PR3 can cleave the complex between these inhibiting ANCA and PR3 itself, leaving active PR3. Autoantibodies to PR3 are potent activators of the 5-lipoxygenase pathway in primed human neutrophils. Extracellular free arachidonic acid, as present at an inflammatory focus, synergizes with such autoantibodies to evoke full-blown lipid mediator generation, granule secretion and respiratory burst. Proteinase 3 (PR3) is a neutral serine proteinase, which is localized in the azurophilic granules of neutrophils and in granules of monocytes and can be detected in the membrane of secretory vesicles. PR3 degrades a number of extracellular matrix proteins such as elastin and inactivates human C1 inhibitor. Membrane-associated PR3 is also able to activate caspase-3 without triggering apoptosis of neutrophils, which is possibly a neutrophil survival mechanism. In addition, PR3 is involved in myeloid differentiation and is, therefore, also called myeloblastin. Monoclonal antibody WGM2 blocks the PR3 activity and partially inhibits the binding of human PR3-ANCA to PR3.