For neutralization of biological activity in vitro dilutions have to be made according to the amounts of -10 TNF-alpha to be inactivated. The affinity of the monoclonal antibody 5N is 5.7 x 10 M. The homologous ELISA is useful for the detection of oligomeric human TNF-alpha. It does not recognize monomeric TNF-alpha.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
PBS, containing 0.1 % bovine serum albumin.
Lagerung
4 °C
Informationen zur Lagerung
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Haltbarkeit
12 months
Song, Fox, Gallo, Rosenberg, Jordan, Shealy, Wagner: "Effects of 2 different anti-tumor necrosis factor-alpha agents in a primate model of subcutaneous abscess formation." in: The Journal of infectious diseases, Vol. 185, Issue 2, pp. 204-13, (2002) (PubMed).
Petyovka, Lyach, Voitenok: "Homologous ELISA for detection of oligomeric human TNF: properties of the assay." in: Journal of immunological methods, Vol. 186, Issue 2, pp. 161-70, (1995) (PubMed).
The monoclonal antibody 5N reacts with Tumor Necrosis Factor alpha (TNF-alpha). Tumor necrosis factor-alpha (TNF-alpha), a homotrimeric 17 KD protein, is a potent mediator of inflammatory and metabolic functions. TNF-alpha was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Further TNF-alpha has been implied as central mediator in shock induced by gram negative micro-organisms. The cytokine TNF-alpha is found to be a central mediator in many inflammatory and immunological processes: it can be induced by various products of micro-organisms and by various cytokines but it also induces on its turn the production of many cytokines. Signal transduction occurs via two types of TNF-receptors, the TNF-receptors I and II. The receptors differ strongly in their intra-cellular signaling pathways. The TNF-alpha trimer interacts with either of the two types of TNF-R leading to receptor cross-linking. The monoclonal antibody 5N cross-reacts and cross-neutralizes all monkey species TNF-alpha, except pig-tail macaque. With affinity about two orders lower it recognizes bovine and canine TNF-alpha. Aliases TNF, TNF-SF2, DIF, Cachectin