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UBE2J1 Antikörper (N-Term)

Rockland 600-401-991 UBE2J1 Reaktivität: Human WB, ELISA, IP Wirt: Kaninchen Polyclonal unconjugated
Rockland
Produktnummer ABIN233822
Hersteller Produkt- Nr.: 600-401-991
  • Target Alle UBE2J1 Antikörper anzeigen
    UBE2J1 (Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1))
    Bindungsspezifität
    • 15
    • 7
    • 3
    • 2
    • 1
    • 1
    N-Term
    Reaktivität
    • 34
    • 7
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Wirt
    • 29
    • 6
    Kaninchen
    Klonalität
    • 30
    • 5
    Polyklonal
    Konjugat
    • 16
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser UBE2J1 Antikörper ist unkonjugiert
    Applikation
    • 33
    • 14
    • 13
    • 13
    • 7
    • 6
    • 3
    • 3
    • 2
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Hersteller Produkt- Nr.
    600-401-991
    Hersteller
    Rockland
    Verwendungszweck
    UBE2J1 Antibody
    Kreuzreaktivität (Details)
    This affinity purified antibody is directed against human Ube2j1 protein.
    Produktmerkmale
    Synonyms: rabbit anti-UBE2J1 antibody, rabbit anti-E2 J1 antibody, rabbit anti-Ubiquitin conjugating enzyme E2J1 antibody, Non-canonical ubiquitin-conjugating enzyme 1, Ube2j1, E2 ubiquitin-conjugating enzyme J1, NCUBE-1, Yeast ubiquitin-conjugating enzyme UBC6 homolog E, HsUBC6e
    Aufreinigung
    The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
    Sterilität
    Sterile filtered
    Immunogen

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near the amino terminus of the human Ube2j1 protein.

    Immunogen Type: Conjugated Peptide

    Isotyp
    IgG
    Top Product
    Discover our top product UBE2J1 Primärantikörper
  • Applikationshinweise

    Application Note: This affinity purified antibody has been tested for use in ELISA, western blotting and immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 35-40 kDa in size corresponding to Ube2j1 protein by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1:200 - 1:2,000

    Immunoprecipitation Dilution: 1-2 μg

    ELISA Dilution: 1:1,000 - 1:5,000

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.71 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Target
    UBE2J1 (Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1))
    Andere Bezeichnung
    UBE2J1 (UBE2J1 Produkte)
    Hintergrund
    Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Ube2j1 and Ube2j2 are homologs of the yeast ubiquitin-conjugating enzyme UBC6, which catalyzes the covalent attachment of ubiquitin to other proteins. These proteins constitute a distinct family of ubiquitin-conjugating enzymes sharing a conserved non-canonical active site sequence and a C-terminal transmembrane domain.  By analogy with yeast UBC6, Ube2j1 and Ube2j2 are localized to the endoplasmic reticulum and seem to function in the selective degradation of misfolded membrane proteins and in general mediation of the stress response.
    Gen-ID
    51465, 37577122
    UniProt
    Q9Y385
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