TDGF1 Antikörper

Produktnummer ABIN233828

Hersteller Produkt- Nr.: 600-401-997

Produktdetails anti-TDGF1 Antikörper

Target: TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TDGF1 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 600-401-997

Hersteller: Rockland

Verwendungszweck: Cripto-1 TDGF1 Antibody

Kreuzreaktivität (Details): This antibody is specific for human Cripto-1 protein.

Produktmerkmale: Synonyms: rabbit anti-Cripto 1 antibody, rabbit anti-Cripto-1 antibody, Cripto 1 growth factor antibody, Epidermal growth factor like cripto protein CR1 antibody, TDGF 1 antibody, Teratocarcinoma-derived growth factor 1, CRGF, TDGF1

Aufreinigung: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human Cripto-1 protein.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 21 kDa in size corresponding to Cripto-1 by western blotting in the appropriate cell lysate or extract.  Cripto-1 is reported as a 188 amino acid protein with a molecular weight of 21.1 kDa.

Western Blot Dilution: 1:1000

ELISA Dilution: 1:105,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.66 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-TDGF1 Antikörper (ABIN233828)

Ligtenberg, Witt, Galvez-Cancino, Sette, Lundqvist, Lladser, Kiessling: "Cripto-1 vaccination elicits protective immunity against metastatic melanoma." in: Oncoimmunology, Vol. 5, Issue 5, pp. e1128613, (2016) (PubMed).

Strizzi, Margaryan, Gerami, Haghighat, Harms, Madonna, Botti, Ascierto, Hendrix: "Translational significance of Nodal, Cripto-1 and Notch4 in adult nevi." in: Oncology letters, Vol. 12, Issue 2, pp. 1349-1354, (2016) (PubMed).

Tysnes, Satran, Mork, Margaryan, Eide, Petersen, Strizzi, Hendrix: "Age-Dependent Association between Protein Expression of the Embryonic Stem Cell Marker Cripto-1 and Survival of Glioblastoma Patients." in: Translational oncology, Vol. 6, Issue 6, pp. 732-41, (2014) (PubMed).

Strizzi, Margaryan, Gilgur, Hardy, Normanno, Salomon, Hendrix: "The significance of a Cripto-1 positive subpopulation of human melanoma cells exhibiting stem cell-like characteristics." in: Cell cycle (Georgetown, Tex.), Vol. 12, Issue 9, pp. 1450-6, (2013) (PubMed).

Malchenko, Galat, Seftor, Vanin, Costa, Seftor, Soares, Hendrix: "Cancer hallmarks in induced pluripotent cells: new insights." in: Journal of cellular physiology, Vol. 225, Issue 2, pp. 390-3, (2010) (PubMed).

Details zu TDGF1

Target: TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))

Andere Bezeichnung: TDGF1 (TDGF1 Produkte)

Hintergrund: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Human Cripto-1 (CR-1), also known as Teratocarcinoma-derived growth factor 1 (TDGF1), is a member of the epidermal growth factor (EGF)-GFC family and has been implicated in both embryogenesis and carcinogenesis.  During early vertebrate development, CR-1 functions as a co-receptor for Nodal, a transforming growth factor b (TGFb) family member, and is essential for mesoderm and endoderm formation and anterior-posterior and left-right axis establishment.  In adult tissues, CR-1 is expressed at a low level in all stages of mammary gland development, and expression increases during pregnancy and lactation.  Over-expression of CR-1 in mouse mammary epithelial cells leads to their transformation in vitro, and when injected in mammary glands, CR-1 produces ductal hyperplasias.

Gen-ID: 6997, 4507425

UniProt: P13385

Pathways: EGFR Signaling Pathway