SIPA1 Antikörper (N-Term)

Produktnummer ABIN233836

Produktdetails anti-SIPA1 Antikörper

Target: SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SIPA1 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Verwendungszweck: Sipa1 Antibody

Kreuzreaktivität (Details): This antibody is specific for mouse Sipa1 protein.

Produktmerkmale: Synonyms: rabbit anti-Sipa1 antibody, Sipa-1, Sipa 1, GTPase activating protein Spa 1 antibody, p130 SPA-1 antibody, Signal induced proliferation associated 1 antibody, SPA1

Aufreinigung: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of mouse Sipa1.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1.25-2.5 μg/mL

Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 130 kDa in size corresponding

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.25 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu SIPA1

Target: SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))

Andere Bezeichnung: Sipa1 (SIPA1 Produkte)

Hintergrund: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Sipa1 (signal-induced proliferation associated gene 1) is a mitogen-induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. Sipa1 is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date.

Gen-ID: 20469

UniProt: P46062

Pathways: Response to Water Deprivation