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SMAD2 Antikörper (Internal Region)

SMAD2 Reaktivität: Human, Affe WB, ELISA, IP Wirt: Kaninchen Polyclonal unconjugated
Rockland
Produktnummer ABIN233848
Hersteller Produkt- Nr.: 600-401-a59
  • Target Alle SMAD2 Antikörper anzeigen
    SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
    Bindungsspezifität
    • 52
    • 44
    • 29
    • 26
    • 24
    • 22
    • 9
    • 9
    • 8
    • 7
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reaktivität
    • 250
    • 161
    • 128
    • 13
    • 10
    • 10
    • 9
    • 8
    • 7
    • 7
    • 7
    • 6
    • 5
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    Human, Affe
    Wirt
    • 247
    • 14
    • 1
    Kaninchen
    Klonalität
    • 224
    • 38
    Polyklonal
    Konjugat
    • 122
    • 17
    • 12
    • 10
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    Dieser SMAD2 Antikörper ist unkonjugiert
    Applikation
    • 205
    • 97
    • 64
    • 54
    • 53
    • 45
    • 41
    • 41
    • 17
    • 15
    • 12
    • 11
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Hersteller Produkt- Nr.
    600-401-a59
    Hersteller
    Rockland
    Verwendungszweck
    SMAD2 Antibody
    Kreuzreaktivität (Details)
    This affinity purified antibody is directed against human Smad2 protein.
    Produktmerkmale
    Synonyms: rabbit anti-SMAD2 antibody, SMAD-2, SMAD 2, mothers against decapentaplegic homolog 2 antibody, MAD homolog 2, Mothers against DPP homolog 2, SMAD family member 2, MADH2, MADH 2, JV18-1
    Aufreinigung
    The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
    Sterilität
    Sterile filtered
    Immunogen

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad2 protein.

    Immunogen Type: Conjugated Peptide

    Isotyp
    IgG
    Top Product
    Discover our top product SMAD2 Primärantikörper
  • Applikationshinweise

    Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 52 kDa in size corresponding to Smad2 protein by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1:1,000 - 1:3,000

    Immunoprecipitation Dilution: User Optimized

    ELISA Dilution: 1:100,000

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.55 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Chung, Overstreet, Li, Wang, Niu, Wang, Fan, Sasaki, Jin, Khodo, Gewin, Zhang, Harris: "TGF-β promotes fibrosis after severe acute kidney injury by enhancing renal macrophage infiltration." in: JCI insight, Vol. 3, Issue 21, (2018) (PubMed).

  • Target
    SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
    Andere Bezeichnung
    SMAD2 (SMAD2 Produkte)
    Hintergrund
    Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI). Smad2 (also known as Mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, Mad2, hMAD-2 or hSMAD2) is a member of the Smad family of proteins which are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively, spliced transcript variants have been observed for this gene. SMAD2 may act as a tumor suppressor in colorectal carcinoma. It positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. SMAD2 may be associated with diseases such as Keloids and Ureteral Disease. Anti-SMAD2 Antibody is useful for researchers interested in SMAD pathways, transcription factor activity, sequence-specific DNA binding, and cancer research.
    Gen-ID
    4087, 5174511
    UniProt
    Q15796
    Pathways
    Zellzyklus, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus
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