EGFR Antikörper (pTyr1173)

Produktnummer ABIN235075

Produktdetails anti-EGFR Antikörper

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Bindungsspezifität: pTyr1173

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EGFR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Indirect Immunofluorescence Assay (IFA)

Spezifität: Binding of EGF to the extracellular domain of the EGF-receptor results in receptor dimerization and autophosphorylation on tyrosine residues, Y1173 being the major autophosphorylation site. Clone 9H2 specifically interacts with the 1170 - NAEpYLRV motif corresponding to the major autophosphorylation site of human EGFR. The antibody does not interact with the non-phosphorylated EGFR nor with unrelated Tyrosine-phosphorylated proteins. This phosphorylation site-specific monoclonal antibody allows the detection and quantification of activated EGFR in crude cell extracts in Immunoblot, ELISA and Biosensor assay formats without the use of P32.

Kreuzreaktivität (Details): Reacts with Human, mouse, and dog.

Produktmerkmale: Cell lysate of pervanadate-treated HepG2 cells, Cell culture

Aufreinigung: Thiophilic adsorption and size exclusion Chromatography. Cell culture

Immunogen: Phosphopeptide conjugated to KLH

Klon: 9H2

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoblotting: 0.5 µg/mL for HRP/ECL detection. Recommended blocking buffer CPPT: 0.5 % (w/v) casein, 5 % (w/v) PEG 4000, 5 % (w/v) Polyvinylpyrrolidone (PVP), 0.5 % Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium Chloride. Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A549 tumor cells were applied to SDS-PAGE (20.000 cells per lane) and transferred to a PVDF membrane. The blot was probed with 0.5- g/mL K67902M for 1 hour at RT and developed by ECL (exp. time: 30 sec). Immunoprecipitation: 1-10ug per 106 vanadate treated A431 cells. Immunocytochemistry: 1-10 µg/mL ELISA: 0.05 µg/mL Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 1 mL water for 15 minutes at room temperature.

Buffer: Lyophilized from PBS, PEG and Sucrose, 0.09 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Store lyophilized product at -20 °C. After reconstitution, aliquot and store at -80 °C. Thawed aliquots may be stored at 2-0 °C for up to 3 months.

Details zu EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Abstract: EGFR Produkte

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteine