Suitable for use in ELISA (1:5.000-1:40.000) and Western blot (1:200-1:5.000). Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute with 0.5 mL distilled water.
Buffer
Lyophilized from 75 mM PBS, pH 7.2 containing 75 mM Sodium chloride, 0.5 mM EDTA and 15 mg/mL BSA, 0.02 % Sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Prepare working dilution only prior to immediate use.The concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol.Centrifuge product if not completely clear after standing for 10 minutes at room temperature. Centrifuge product if not completely clear after standing at room temperature. Prepare working dilution only prior to immediate use.
Lagerung
4 °C
Informationen zur Lagerung
Store lyophilized product at 2-8 °C. After reconstitution, product is stable for several weeks at 2-8 °C as an undiluted liquid. For extended storage after reconstitution, we suggest the addition of an equal volume of glycerol to make a final glycerol concentration of 50 % followed by storage at -28 °C.