Borrelia Burgdorferi Antikörper (FITC)

Produktnummer ABIN236886

Produktdetails anti-Borrelia Burgdorferi Antikörper (FITC)

Target: Borrelia Burgdorferi

Reaktivität: Borrelia burgdorferi

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Borrelia Burgdorferi Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: Reactive Western blot bands at 83kD, 41kD, 34kD and 31kD and additional low MW bands. Cross-reacts with Treponema pallidum, B. hermsii & B. parkerii.

Produktmerkmale: Rabbit anti B. Burgdorferi, Rabbit antibody to Borrelia burgdorferi (Lyme) Fluorescein conjugated

Aufreinigung: Covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

Immunogen: Whole cell preparation from B. burgdorferi.

Anwendungsinformationen

Applikationshinweise: Suitable for use in direct IFA, Western blot and immunohistochemistry (formalin fixed/paraffin). Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Protocol for direct IFA: 1. Multi-well slide contains acetone fixed organisms (BION Enterprises). 2. 2-fold dilutions of ABIN236886 starting at 1:10 out to 1:80 with a counterstain blocking diluents (Evan’s Blue in BSA solution). 3. Incubate 30 minutes at Room Temperature. 4. Wash 5. Mounting medium added to each well before coverslipping the slide. 6. Typically observe 4+ staining for several of the dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.01 M PBS, pH7.2 containing 10 mg/mL BSA, 0.1 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid multiple freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store (up to 6 months) at 2-8 °C under subdued light. Long term, aliquot and store at -28 °C.

Haltbarkeit: 6 months

Details zu Borrelia Burgdorferi

Target: Borrelia Burgdorferi

Andere Bezeichnung: Borrelia Burgdorferi (Lyme) (Borrelia Burgdorferi Produkte)