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CK1 epsilon Antikörper (C-Term)

Verified CSNK1E Reaktivität: Human WB, ELISA, IHC, IF, FACS Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN238540
  • Target Alle CK1 epsilon (CSNK1E) Antikörper anzeigen
    CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))
    Bindungsspezifität
    • 13
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 64
    • 39
    • 19
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 61
    • 8
    • 2
    Ziege
    Klonalität
    • 63
    • 8
    Polyklonal
    Konjugat
    • 39
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Dieser CK1 epsilon Antikörper ist unkonjugiert
    Applikation
    • 48
    • 33
    • 30
    • 14
    • 11
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    Verwendungszweck
    CSNK1E
    Sequenz
    PASQTSVPFD HLGK
    Kreuzreaktivität
    Hund, Human, Maus, Ratte
    Aufreinigung
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Güteklasse
    Verified
    Immunogen
    Peptide with sequence C-PASQTSVPFDHLGK, from the C Terminus of the protein sequence according to NP_001885.1, NP_689407.1.
    Isotyp
    IgG
  • Applikationshinweise

    Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 48 kDa band observed in nuclear lysates of cell lines A431and HeLa, and approx 45 kDa in nuclear lysates of cell line K562 (calculated MW of 47.3 kDa according to NP_001276841.1). Recommended concentration: 0.3-1 μg/mL. Primary incubatio

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Kommentare

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Minimize freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))
    Andere Bezeichnung
    CSNK1E (CSNK1E Produkte)
    Hintergrund
    CSNK1E, casein kinase 1, epsilon, RP1-5O6.1, HCKIE, MGC10398 , CKI-epsilon, OTTHUMP00000063258, OTTHUMP00000063263, casein kinase 1 epsilon, casein kinase I epsilon
    Gen-ID
    1454, 27373, 58822
    NCBI Accession
    NP_001276841
    Pathways
    Hedgehog Signalweg, M Phase
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