CK1 epsilon Antikörper (C-Term)
-
- Target Alle CK1 epsilon (CSNK1E) Antikörper anzeigen
- CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))
-
Bindungsspezifität
- C-Term
-
Reaktivität
- Human
-
Wirt
- Ziege
-
Klonalität
- Polyklonal
-
Konjugat
- Dieser CK1 epsilon Antikörper ist unkonjugiert
-
Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Verwendungszweck
- CSNK1E
- Sequenz
- PASQTSVPFD HLGK
- Kreuzreaktivität
- Hund, Human, Maus, Ratte
- Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Güteklasse
- Verified
- Immunogen
- Peptide with sequence C-PASQTSVPFDHLGK, from the C Terminus of the protein sequence according to NP_001885.1, NP_689407.1.
- Isotyp
- IgG
-
-
- Applikationshinweise
-
Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 5 μg/mL.
Western Blot: Approx 48 kDa band observed in nuclear lysates of cell lines A431and HeLa, and approx 45 kDa in nuclear lysates of cell line K562 (calculated MW of 47.3 kDa according to NP_001276841.1). Recommended concentration: 0.3-1 μg/mL. Primary incubatio
Peptide ELISA: antibody detection limit dilution 1:32000.
- Kommentare
-
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Minimize freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
- Target
- CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))
- Andere Bezeichnung
- CSNK1E (CSNK1E Produkte)
- Hintergrund
- CSNK1E, casein kinase 1, epsilon, RP1-5O6.1, HCKIE, MGC10398 , CKI-epsilon, OTTHUMP00000063258, OTTHUMP00000063263, casein kinase 1 epsilon, casein kinase I epsilon
- Gen-ID
- 1454, 27373, 58822
- NCBI Accession
- NP_001276841
- Pathways
- Hedgehog Signalweg, M Phase
-