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WISP1 Antikörper (C-Term)

Verified WISP1 Reaktivität: Human WB, ELISA, IF, FACS Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN238610
  • Target Alle WISP1 Antikörper anzeigen
    WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
    Bindungsspezifität
    • 15
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 40
    • 26
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 55
    • 5
    • 1
    Ziege
    Klonalität
    • 57
    • 4
    Polyklonal
    Konjugat
    • 26
    • 9
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser WISP1 Antikörper ist unkonjugiert
    Applikation
    • 35
    • 21
    • 19
    • 13
    • 13
    • 8
    • 5
    • 5
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Verwendungszweck
    WISP1
    Sequenz
    ESYPDFSEIA N
    Spezifität
    This antibody is expected to recognize both reported isoforms (NP_003873.1, NP_543028.1).
    Kreuzreaktivität
    Human, Maus, Ratte
    Aufreinigung
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Güteklasse
    Verified
    Immunogen
    Peptide with sequence C-ESYPDFSEIAN, from the C Terminus of the protein sequence according to NP_003873.1, NP_543028.1.
    Isotyp
    IgG
  • Applikationshinweise

    Western Blot: Approx 32-35 kDa band observed in lysates of cell line CaCo-2 and approx. 40 kDa in Human Tonsil lysates, and additionally in a lysate of cell line CaCo-2, which were successfully blocked by incubation with the immunising peptide (calculated

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Kommentare

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Minimize freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
    Andere Bezeichnung
    CCN4 (WISP1 Produkte)
    Hintergrund
    WISP1, WNT1 inducible signaling pathway protein 1, CCN4, WISP1c, WISP1i, WISP1tc, WNT1 induced secreted protein 1, Wnt-1 inducible signaling pathway protein 1, wnt-1 signaling pathway protein 1
    Gen-ID
    8840, 22402, 65154
    NCBI Accession
    NP_003873, NP_543028
    Pathways
    WNT Signalweg, Growth Factor Binding
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