Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Sequenz
YPYDVPDYA
Spezifität
Recognizes HA tagged (YPYDVPDYA) proteins overexpressed in cells, including both amino or carboxy-termini of targeted proteins in transfected mammalian cells.
Aufreinigung
Protein A purified
Immunogen
Synthetic peptide containing the sequence aa98-106 (YPYDVPDYA) derived from the influenza virus hemaglutinin (HA) protein (KLH).
Type of Immunogen: Synthetic peptide - KLH conjugated
Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 0.5-2 μg/mL. Detects recombinant protein containing the HA-tag at the N-terminus in RIPA lysates from HEK293 cells. Immunoprecipitation: 5 μg/mL. Reported to immunoprecipitate recombinant HA-tagged fusion protein. Immunocytochemistry: 5 μg/mL. Reported to immunostain HA-tagged fusion protein.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
Lot specific
Buffer
PBS, pH 7.4, 0.05 % sodium azide, 40 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeat freeze-thaw cycles.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.