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5-Methylcytosine Antikörper (FITC)

IF, ICC Wirt: Maus Monoclonal 5MC-CD FITC
Produktnummer ABIN2451914
  • Target
    5-Methylcytosine
    Reaktivität
    Bitte anfragen
    Wirt
    • 22
    • 8
    • 3
    Maus
    Klonalität
    • 24
    • 9
    Monoklonal
    Konjugat
    • 17
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    FITC
    Applikation
    • 20
    • 16
    • 13
    • 10
    • 8
    • 8
    • 7
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Immunocytochemistry (ICC)
    Aufreinigung
    Purified
    Sterilität
    Sterile filtered
    Immunogen
    5-Methylcytosine conjugated to bovine serum albumin
    Klon
    5MC-CD
    Isotyp
    IgM
  • Applikationshinweise
    1) Immunocytochemistry: ~50-100 fold dilution
    2) Immuno-blotting detection of DNA with 5-methylcytosine on nitrocellulose: ~1000 fold dilution
    Not tested with other application
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS with 50 % glycerol
    Konservierungsmittel
    Azide free
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon arrival centrifuge briefly and store at -20 C.
  • Sharif, Muto, Takebayashi, Suetake, Iwamatsu, Endo, Shinga, Mizutani-Koseki, Toyoda, Okamura, Tajima, Mitsuya, Okano, Koseki: "The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA." in: Nature, Vol. 450, Issue 7171, pp. 908-12, (2007) (PubMed).

    Nishiyama, Ito, Yamaguchi, Koizumi, Sano: "A chloroplast-resident DNA methyltransferase is responsible for hypermethylation of chloroplast genes in Chlamydomonas maternal gametes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 9, pp. 5925-30, (2002) (PubMed).

    Sano, Imokawa, Sager: "Detection of heavy methylation in human repetitive DNA subsets by a monoclonal antibody against 5-methylcytosine." in: Biochimica et biophysica acta, Vol. 951, Issue 1, pp. 157-65, (1988) (PubMed).

    Sano, Royer, Sager: "Identification of 5-methylcytosine in DNA fragments immobilized on nitrocellulose paper." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 77, Issue 6, pp. 3581-5, (1980) (PubMed).

  • Target
    5-Methylcytosine
    Andere Bezeichnung
    5 Methylcytosine
    Substanzklasse
    Chemical
    Hintergrund
    DNA methylation is a type of chemical modification of DNA that can be inherited and subsequently removed without changing the original DNA sequence. Therefore it is part of the epigenetic code and is also the most well characterized epigenetic mechanism. DNA methylation results in addition of a methyl group to DNA - for example, to the number 5 carbon of the cytosine pyrimidine ring - which involves reduction in gene expression. In adult somatic tissues, DNA methylation typically occurs in a CpG dinucleotide context, non-CpG methylation is prevalent in embryonic stem cells. This hybridoma has been constructed by Prof. H. Sano.
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