5-Methylcytosine Antikörper (FITC)

Produktnummer ABIN2451914

Produktdetails

Target: 5-Methylcytosine

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: FITC

Applikation: Immunofluorescence (IF), Immunocytochemistry (ICC)

Aufreinigung: Purified

Sterilität: Sterile filtered

Immunogen: 5-Methylcytosine conjugated to bovine serum albumin

Klon: 5MC-CD

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: 1) Immunocytochemistry: ~50-100 fold dilution
2) Immuno-blotting detection of DNA with 5-methylcytosine on nitrocellulose: ~1000 fold dilution
Not tested with other application

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS with 50 % glycerol

Konservierungsmittel: Azide free

Lagerung: -20 °C

Informationen zur Lagerung: Upon arrival centrifuge briefly and store at -20 C.

Referenzen

Sharif, Muto, Takebayashi, Suetake, Iwamatsu, Endo, Shinga, Mizutani-Koseki, Toyoda, Okamura, Tajima, Mitsuya, Okano, Koseki: "The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA." in: Nature, Vol. 450, Issue 7171, pp. 908-12, (2007) (PubMed).

Nishiyama, Ito, Yamaguchi, Koizumi, Sano: "A chloroplast-resident DNA methyltransferase is responsible for hypermethylation of chloroplast genes in Chlamydomonas maternal gametes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 9, pp. 5925-30, (2002) (PubMed).

Sano, Imokawa, Sager: "Detection of heavy methylation in human repetitive DNA subsets by a monoclonal antibody against 5-methylcytosine." in: Biochimica et biophysica acta, Vol. 951, Issue 1, pp. 157-65, (1988) (PubMed).

Sano, Royer, Sager: "Identification of 5-methylcytosine in DNA fragments immobilized on nitrocellulose paper." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 77, Issue 6, pp. 3581-5, (1980) (PubMed).

Antigendetails

Target: 5-Methylcytosine

Andere Bezeichnung: 5 Methylcytosine

Substanzklasse: Chemical

Hintergrund: DNA methylation is a type of chemical modification of DNA that can be inherited and subsequently removed without changing the original DNA sequence. Therefore it is part of the epigenetic code and is also the most well characterized epigenetic mechanism. DNA methylation results in addition of a methyl group to DNA - for example, to the number 5 carbon of the cytosine pyrimidine ring - which involves reduction in gene expression. In adult somatic tissues, DNA methylation typically occurs in a CpG dinucleotide context, non-CpG methylation is prevalent in embryonic stem cells. This hybridoma has been constructed by Prof. H. Sano.