Fc epsilon RI/FCER1A Antikörper (AA 85-172) (FITC)

Produktnummer ABIN2451978

Produktdetails anti-Fc epsilon RI/FCER1A Antikörper (FITC)

Target: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Bindungsspezifität: AA 85-172

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Fc epsilon RI/FCER1A Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktmerkmale: The IgG fraction was purified from serum free culture medium of mouse hybridoma (CRA2) by propriety chromatography under mild conditions.

Aufreinigung: Purified

Sterilität: Sterile filtered

Immunogen: AA 85-172 of FcepsilonR1alpha

Klon: CRA2

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: 1) FACS
2) Immunohistochemistry and immunocytochemistry
3) Inhibition of binding of IgE with FcepsilonR1alpha.
Titration of IgE-bound fraction of the FcepsilonR1alpha using CRA1 and CRA2 antibodies

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS ( pH 7.4), 50 % glycerol

Konservierungsmittel: Azide free

Lagerung: -20 °C

Referenzen für anti-Fc epsilon RI/FCER1A Antikörper (FITC) (ABIN2451978)

Takai, Yuuki, Iwamoto-Yasue, Okumura, Ra: "Epitope analysis and primary structures of variable regions of anti-human FcepsilonRI monoclonal antibodies, and expression of the chimeric antibodies fused with human constant regions." in: Bioscience, biotechnology, and biochemistry, Vol. 64, Issue 9, pp. 1856-67, (2001) (PubMed).

Takai, Okumura, Ra: "Direct expression of the extracellular portion of human FcepsilonRIalpha chain as inclusion bodies in Escherichia coli." in: Bioscience, biotechnology, and biochemistry, Vol. 65, Issue 1, pp. 79-85, (2001) (PubMed).

Goto, Soma, Ra, Kawa, Kubota, Mizoguchi: "Enhanced expression of the high-affinity receptor for IgE (Fc(epsilon)RI) associated with decreased numbers of Langerhans cells in the lesional epidermis of atopic dermatitis." in: Journal of dermatological science, Vol. 27, Issue 3, pp. 156-61, (2001) (PubMed).

Hasegawa, Pawankar, Suzuki, Nakahata, Furukawa, Okumura, Ra: "Functional expression of the high affinity receptor for IgE (FcepsilonRI) in human platelets and its' intracellular expression in human megakaryocytes." in: Blood, Vol. 93, Issue 8, pp. 2543-51, (1999) (PubMed).

Hakimi, Seals, Kondas, Pettine, Danho, Kochan: "The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding." in: The Journal of biological chemistry, Vol. 265, Issue 36, pp. 22079-81, (1991) (PubMed).

Ra, Jouvin, Blank, Kinet: "A macrophage Fc gamma receptor and the mast cell receptor for IgE share an identical subunit." in: Nature, Vol. 341, Issue 6244, pp. 752-4, (1989) (PubMed).

Details zu Fc epsilon RI/FCER1A

Target: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Abstract: FCER1A Produkte

Synonyme: FCER1A antikoerper, FCE1A antikoerper, FcERI antikoerper, Iger01 antikoerper, RATIGER01 antikoerper, Fce1a antikoerper, Fcr-5 antikoerper, fcepsilonri antikoerper, Fc fragment of IgE receptor Ia antikoerper, Fc receptor, IgE, high affinity I, alpha polypeptide antikoerper, FCER1A antikoerper, Fcer1a antikoerper

Hintergrund: FcεR1alpha is subunit of the high affinity receptor for IgE to which IgE directly binds. FcεR1alpha is a tetrameric complex consisting of one alpha, one beta and two γ subunits. The latter two are required for signal transduction activity. The FcεR1 complex plays an important role in triggering allergic responses. The CRA2 (AER24) monoclonal antibody reacts with the FcεR1alpha subunit on a region that overlaps the region of the IgE binding site, thus it competes with IgE for the receptor binding. Since the CRA1 (AER37) monoclonal antibody reacts with the site different from the IgE binding site on FcεR1alpha, it does not compete with IgE for the receptor binding. Combining the two antibodies, one can quantitatively measure the amounts of the IgE-bound FcεR1alpha.

UniProt: P12319

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process