HBEGF Antikörper (EGF Like Domain) (Biotin)

Produktnummer ABIN2451994

Produktdetails anti-HBEGF Antikörper (Biotin)

Target: HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))

Bindungsspezifität: EGF Like Domain

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HBEGF Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Kreuzreaktivität (Details): Does not react with mouse.

Produktmerkmale: biotin/IgG = 7.5

Aufreinigung: Purified

Sterilität: Sterile filtered

Immunogen: Recombinant human HB-EGF ectodomain expressed in SF21 cell

Klon: 4G10

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: 1) Western blotting: 0. 2~1 g/mL
2) Immunoprecipitation: 2 g/mL
3) Indirect immuno-fluorescence staining: 5~10 g/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol

Konservierungsmittel: Azide free

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: -20 C (For long term storage: -80 C)

Referenzen für anti-HBEGF Antikörper (Biotin) (ABIN2451994)

Miyamoto, Hirata, Yamazaki, Kageyama, Hasuwa, Mizushima, Tanaka, Yagi, Sonoda, Kai, Kanoh, Nakano, Mekada: "Heparin-binding EGF-like growth factor is a promising target for ovarian cancer therapy." in: Cancer research, Vol. 64, Issue 16, pp. 5720-7, (2004) (PubMed).

Prenzel, Zwick, Daub, Leserer, Abraham, Wallasch, Ullrich: "EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF." in: Nature, Vol. 402, Issue 6764, pp. 884-8, (2000) (PubMed).

Iwamoto, Higashiyama, Mitamura, Taniguchi, Klagsbrun, Mekada et al.: "Heparin-binding EGF-like growth factor, which acts as the diphtheria toxin receptor, forms a complex with membrane protein DRAP27/CD9, which up-regulates functional receptors and diphtheria toxin ..." in: The EMBO journal, Vol. 13, Issue 10, pp. 2322-30, (1994) (PubMed).

Higashiyama, Abraham, Miller, Fiddes, Klagsbrun: "A heparin-binding growth factor secreted by macrophage-like cells that is related to EGF." in: Science (New York, N.Y.), Vol. 251, Issue 4996, pp. 936-9, (1991) (PubMed).

Details zu HBEGF

Target: HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))

Andere Bezeichnung: HB-EGF (HBEGF Produkte)

Synonyme: hbegf antikoerper, zgc:165443 antikoerper, HBEGF antikoerper, zgc:175091 antikoerper, DTR antikoerper, DTS antikoerper, DTSF antikoerper, HEGFL antikoerper, Dtr antikoerper, GFHB antikoerper, Hb-egf antikoerper, Hegfl antikoerper, AW047313 antikoerper, Dts antikoerper, HB-EGF antikoerper, heparin-binding EGF-like growth factor b antikoerper, heparin binding EGF like growth factor antikoerper, Heparin-binding EGF-like growth factor antikoerper, heparin-binding EGF-like growth factor a antikoerper, heparin-binding EGF-like growth factor antikoerper, hbegfb antikoerper, HBEGF antikoerper, hbegf antikoerper, hbegfa antikoerper, Hbegf antikoerper

Hintergrund: Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is synthesized as a membrane-anchored precursor that is proteolytically cleaved to release the soluble mature growth factor, HB-EGF. The former functions as juxtacrine and the latter as paracrine growth factor. Soluble HB-EGF shows several forms in Western blotting with apparent molecular weights 19~27 kDa due to heterogeneous O-glycosylation and N-terminal truncation. HB-EGF activates EGFR and ErbB4 and promotes the development in many tissues. In human ProHB-EGF is the cellular receptor for diphtheria toxin. Non-toxic mutant of diphtheria toxin, CRM197, inhibits HB-EGF function. As HB-EGF level is elevated in most ovarian cancer, CRM197 is being tested as an anticancer drug. The hybridoma clone 4G10 was established and characterized in the laboratory of Prof. E. Mekada of Osaka University, who is a leading scientist in this field.

UniProt: Q99075

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung