HIV-1 p24 Antikörper (Biotin)

Produktnummer ABIN2452028

Produktdetails anti-HIV-1 p24 Antikörper (Biotin)

Target: HIV-1 p24 (Human Immunodeficiency Virus 1 Capsid (HIV-1 p24))

Reaktivität: Human Immunodeficiency Virus (HIV)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HIV-1 p24 Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Produktmerkmale: This product is a biotinylated IgG ([biotin]/[IgG] = 8.0) produced from the purified IgG fraction of rabbit anti-p24 serum.

Aufreinigung: Purified

Sterilität: Sterile filtered

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: 1. Western blotting
2. Immunoprecipitaiton
3. Immunofluorescence staining
4. ELISA
Not tested in other application

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.9 mg/mL

Buffer: PBS, 50 % glycerol

Konservierungsmittel: Azide free

Lagerung: -20 °C

Referenzen für anti-HIV-1 p24 Antikörper (Biotin) (ABIN2452028)

Freed: "HIV-1 gag proteins: diverse functions in the virus life cycle." in: Virology, Vol. 251, Issue 1, pp. 1-15, (1998) (PubMed).

Saito, Morimoto, Ohara, Takamizawa, Nakata, Shinagawa: "Overproduction, purification, and diagnostic use of the recombinant HIV-1 Gag proteins, the precursor protein p55 and the processed products p17, p24, and p15." in: Microbiology and immunology, Vol. 39, Issue 7, pp. 473-83, (1996) (PubMed).

Tanaka, Saitoh, Nakata, Shinagawa: "A simple method for overproduction and purification of p24 Gag protein of human immunodeficiency virus type 1." in: Microbiology and immunology, Vol. 36, Issue 8, pp. 823-31, (1993) (PubMed).

Adachi, Gendelman, Koenig, Folks, Willey, Rabson, Martin: "Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone." in: Journal of virology, Vol. 59, Issue 2, pp. 284-91, (1986) (PubMed).

Details zu HIV-1 p24

Target: HIV-1 p24 (Human Immunodeficiency Virus 1 Capsid (HIV-1 p24))

Andere Bezeichnung: HIV-1 p24 (HIV-1 p24 Produkte)

Substanzklasse: Viral Protein

Hintergrund: HIV-1 Gag p24 is a capsid protein that constitutes the core of AIDS virus HIV-1 and is produced by digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the reproduction of AIDS virus and constitutes an essential element for the AIDS virus particle construction. p24 is used as a marker antigen for observing the patient's condition after treatment, as it indicates the amount of virus in the blood. The product is prepared by immunizing rabbit with recombinant p24 protein which was over-expressed in E. coli with a plasmid carrying the Gag p24 coding region of HIV-1 virus, subtype B, and was highly purified by several steps of chromatography. Using this antiserum in Western blotting, the bands of 24 kD, 55 kD and 41 kD corresponding respectively to HIV-p24 and its precursors p55 and p41 were observed in the extract of the AIDS virus infected cells.