Yamagishi, Sakuno, Shimura, Watanabe: "Heterochromatin links to centromeric protection by recruiting shugoshin." in: Nature, Vol. 455, Issue 7210, pp. 251-5, (2008) (PubMed).
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Kitajima, Kawashima, Watanabe: "The conserved kinetochore protein shugoshin protects centromeric cohesion during meiosis." in: Nature, Vol. 427, Issue 6974, pp. 510-7, (2004) (PubMed).
Shugoshin 1 (Sgo1), a meiosis-specific protein, protects centromeric Rec8 from degradation during first meiotic division (meiosis I) in fission yeast Schizosaccharomyces pombe. This protein appears at late prophase of meiosis I and is degraded at anaphase I. Heterochromatin protein Swi6 associates directly with Sgo1. A point mutation of Sgo1 (V242E), which abolishes the interaction with Swi6, impairs the centromeric localization and function of Sgo1. The forced centromeric localization of Sgo1 restores proper meiotic chromosome segregation in swi6 cells. Using this antibody in Western blotting, the band of 37 kD was observed in wild type S. pombe MP111 extract.