VRK1 Antikörper (N-Term)

Produktnummer ABIN2452161

Produktdetails anti-VRK1 Antikörper

Target: VRK1 (Vaccinia Related Kinase 1 (VRK1))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VRK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Spezifität: Reacts with human VRK1 protein.

Kreuzreaktivität (Details): Not tested with other species.

Produktmerkmale: Mouse monoclonal antibody (5D1) produced in serum-free medium and purified by propriety chromatography under mild conditions (90~98 % pure). Isotype,IgG1 kappa

Sterilität: Sterile filtered

Immunogen: Synthetic peptide corresponding to N-terminus of human VRK1,MPRVKAAQAGRQSSAKRHL-C

Klon: 5D1

Isotyp: IgG2 kappa

Anwendungsinformationen

Applikationshinweise: 1. Western blotting: 1/200~1/1,000 dilution. Use of highly sensitive chemiluminescence reagents such as Lumi-Light Plus (Roche) or ImmunoStaroR LD (Wako,Tokyo) are recommended.
2. Immunoprecipitation (assay dependent)
3. Immunofluorescence staining: 1/100 dilution
4. Immunohistochemistry (assay dependent)
5. ELISA (assay dependent)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS with 50 % Glycerol

Konservierungsmittel: Azide free

Lagerung: -20 °C

Details zu VRK1

Target: VRK1 (Vaccinia Related Kinase 1 (VRK1))

Andere Bezeichnung: VRK1 (VRK1 Produkte)

Synonyme: vrk1 antikoerper, MGC64390 antikoerper, MGC75762 antikoerper, VRK1 antikoerper, PCH1 antikoerper, PCH1A antikoerper, 51PK antikoerper, wu:fe16f06 antikoerper, zgc:56266 antikoerper, vaccinia related kinase 1 L homeolog antikoerper, vaccinia related kinase 1 antikoerper, Serine/threonine-protein kinase VRK1 antikoerper, vrk1.L antikoerper, vrk1 antikoerper, VRK1 antikoerper, vrk-1 antikoerper, Vrk1 antikoerper

Hintergrund: Function, The VRK1 gene encodes serine/threonine kinase VRK1 (Vaccinia-Related Kinase 1, 396 aa, 45.5 kDa) which is involved in Golgi disassembly during the cell cycle following phosphorylation by PLK3 during mitosis, and required to induce Golgi fragmentation. It acts by mediating phosphorylation of a downstream target protein 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. It also phosphorylates casein and histone H3. Phosphorylation of the BANF1 gene product disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Involvement in disease Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1A (PCH1A), also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1A is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy

Gen-ID: 7443

UniProt: Q99986