Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Spezifität
This antibody is expected to recognize both reported isoforms (NP_057065.2, NP_741960.1).
Aufreinigung
CAMK2A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
CAMK2A antibody was raised against a 14 amino acid synthetic peptide near the internal region of CAMK2A.
CAMK2A
Reaktivität: Human
WB, ELISA
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 55+65 kDa bands observed in human cerebellum and mouse brain lysates (calculated MW of 55.3 kDa according to human NP_057065.2 and of 54.1 according to human NP_741960.1 and mouse NP_803126.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human brain cortex shows granular staining in the periphery of pyramydal cell bodies. Recommended concentration, 3-6 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Minimize freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot and store at -20 °C.
Target
CAMK2A
(Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))