PALLD antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
PALLD antibody was raised against a 13 amino acid synthetic peptide near the internal region of PALLD.
ELISA: Antibody detection limit dilution 1:2000. Western Blot: Approximately 125 kDa band observed in human Umbillical Cord lysates (calculated MW of122 kDa according to NP_057165.3). Recommended concentration: 0.1-0.3 μg/mL. an additional band of 55 kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western Blot.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Minimize freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot and store at -20 °C.
Target
PALLD
(Palladin, Cytoskeletal Associated Protein (PALLD))