This antibody is expected to recognize reported isoforms a (NP_665906.1, NP_665908.1, NP_665911.1) and b (NP_002122.1, NP_665910.1, NP_665912.1). This antibody is not expected to cross-react with HMGA2.
Aufreinigung
HMGA1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
HMGA1 antibody was raised against a 13 amino acid synthetic peptide near the internal region (near N-Terminus) of HMGA1 (near N-Terminus).
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 24 kDa band observed in human pancreas (calculated MW of 11.7 kDa /10.7 kDa according to NP_665906.1, NP_002122.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.5-1 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.