Western Blotting (WB), ELISA, Immunoprecipitation (IP)
Aufreinigung
CD244 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
CD244 antibody was raised against a 12 amino acid synthetic peptide near the internal region of CD244.
CD244
Reaktivität: Human
WB
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 75 kDa band observed in human Natural Killer (NK) cell lysates after immunoprecipitation with mouse monoclonal antibody C1.7 coupled to Protein G agarose (calculated MW of 41.1 kDa according to NP_057466.1). We do note that CD244 is a glycoprotein which makes a higher size band to be expected. Recommended concentration: 1-3 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.