ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Aufreinigung
PEBP4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
PEBP4 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of PEBP4.
PEBP4
Reaktivität: Human
ELISA, WB
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 26 kDa band observed in human heart lysates (calculated MW of 25.7 kDa according to NP_659399.2). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 30 kDa . This band was successfully blocked by incubation with the immunizing peptide. IHC: In paraffin embedded Human Pancreas shows staining consistent with lysosomes. Recommended concentration, 5-10μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Minimize freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot and store at -20 °C.
Target
PEBP4
(Phosphatidylethanolamine-Binding Protein 4 (PEBP4))