Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Aufreinigung
FOXI3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
FOXI3 antibody was raised against a 14 amino acid synthetic peptide near the internal region of FOXI3.
FOXI3
Reaktivität: Human
WB, ELISA, FACS
Wirt: Kaninchen
Polyclonal
FITC
Applikationshinweise
ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 50 kDa band in human skin lysates after 1 μg/mL antibody staining . The 50 kDa band was successfully blocked by incubation with the immunizing peptide. IHC: In paraffin embedded Human Bronchus shows strong nuclear staining in the basal cells of the resporatory epithelium. Recommended concentration, 5-10μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.