TGFBI antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
TGFBI antibody was raised against a 13 amino acid synthetic peptide near the Internal region of TGFBI.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 70 kDa band observed in human, mouse and rat kidney lysates (calculated MW of 74.7 kDa according to human NP_000349.1). Recommended concentration: 0.2-0.5 μg/mL. In paraffin embedded kidney shows staining of intercalated cells of a collecting tubule. Recommended concentration, 4-6 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.