Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
Spezifität
This antibody is expected to recognize isoform 1 (NP_073753.3) only.
Aufreinigung
CDCP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
CDCP1 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of CDCP1.
CDCP1
Reaktivität: Human
WB
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 90 kDa band observed in human colon lysates (calculated MW of 92.3 kDa according to NP_073753.3). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 200 kDa . This band was successfully blocked by incubation with the immunizing peptide. Immunoprecipitation: This antibody has been successfully used in IP from A549 cells.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.