Western Blotting (WB), ELISA, Immunoprecipitation (IP)
Spezifität
This antibody is expected to recognize both reported isoforms NP_004304.1 and NP_945355.1. No crossreactivity is expected with Arrestin beta 1.
Aufreinigung
Arrestin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Arrestin antibody was raised against an 11 amino acid synthetic peptide near the internal region of Arrestin.
SAG
Reaktivität: Human
WB, ELISA, IHC
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 40 kDa band observed in human brain lysates (calculated MW of 46.1 kDa according to NP_004304.1 and 44.4 according to NP_945355.1). Recommended concentration: 0.3-1 μg/mL. Immunohistochemistry: In paraffin embedded human cerebellum shows mainly nuclear staining of Purkinje cells and neuronal cells in the Molecular Layer. Recommended concentration, 4-6 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Minimize freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot and store at -20 °C.
Target
SAG
(S-Antigen, Retina and Pineal Gland (Arrestin) (SAG))