Cathepsin F antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Cathepsin F antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of Cathepsin F.
Verified
CTSF
Reaktivität: Human
WB, ELISA
Wirt: Ziege
Polyclonal
unconjugated
Applikationshinweise
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: experiments gave bands at approximately 30 kDa and 35 kDa in human prostate and ovary lysates after 0.3 μg/mL antibody staining. These bands correspond to earlier findings in literature with different antibodies (Wang et al, J Biol Chem. 1998 Nov 27,273 (48) :32000-8, PMID: 9822672). This protein has a calculated MW of 53.4 kDa according to NP_003784.2. Recommended concentration: 0.3-1 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.