EDD1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
EDD1 antibody was raised against an 11 amino acid synthetic peptide near the C-Terminus of EDD1.
ELISA: Antibody detection limit dilution >1:32000. Western Blot: Approximately 35-40 kDa band observed in human testis, human muscle, A549 and A431 lysates at 0.5-1 ?of primary, this band was successfully blocked by incubation with the immunizing peptide. Please note that the band we observe is different from the predicted size of approximately 322 kDa according to NP_056986. Immunohistochemistry: In paraffin embedded human testis shows nuclear staining in . Recommended concentration, 1-2 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Minimize freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot and store at -20 °C.
Target
UBR5
(Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5))