This antibody is expected to recognize both reported isoforms (NP_862820.1 and NP_036513.2).
Aufreinigung
ORC3L antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ORC3L antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of ORC3L.
ORC3
Reaktivität: Human
WB, IHC (p)
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 75 kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 82.3 kDa according to NP_862820.1 and NP_036513.2). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 35 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.