Parvalbumin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Parvalbumin antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of Parvalbumin.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 12 kDa band observed in human brain lysates (calculated MW of 12.1 kDa according to NP_002845.1). Recommended concentration: 0.1-0.3 μg/mL. Approximately 10-12 kDa band observed in rat skeletal muscle and in rat heart cells transfected with human PVALB, but not in untransfected rat heart cells. Data kindly provided by Dr Wang Wang, University of Michigan
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.