S3-12 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
S3-12 antibody was raised against a 16 amino acid synthetic peptide near the internal region of S3-12.
PLIN4
Reaktivität: Human
IHC, WB, ICC, IF
Wirt: Meerschweinchen
Polyclonal
unconjugated
Applikationshinweise
ELISA: Antibody detection limit dilution 1:2,000. Western Blot: Approximately 45 kDa and 50 kDa in human adipose lysates after 0.1 μg/mL antibody staining.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Immunohistochemistry: In paraffin embedded human pancreas shows membranous and cytoplasm staining in adipocytes. Recommended concentration, 3-6 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.