Spinesin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Spinesin antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of Spinesin.
TMPRSS5
Reaktivität: Human
WB, IF (cc), IF (p)
Wirt: Kaninchen
Polyclonal
AbBy Fluor® 647
Applikationshinweise
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: experiments gave bands at approximately 50 kDa and 29 kDa in human cerebral cortex lysates and in mouse and rat brain lysates after 0.5 μg/mL antibody staining. These bands correspond to earlier findings in literature with different antibodies (Yamaguchi et al, J Biol Chem. 2002 Mar 1,277 (9) :6806-12, PMID: 11741986). This protein has a calculated MW of 49.6 kDa according to NP_110397.1. Recommended concentration: 0.5-1.5 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.