This antibody is expected to recognize both reported isoforms (NP_071320.1 and NP_071505.1).
Aufreinigung
TIA1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
TIA1 antibody was raised against an 11 amino acid synthetic peptide near the internal region of TIA1.
TIA1
Reaktivität: Human
WB, IF
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 40 kDa band observed in Jurkat lysate (calculated MW of 41.8 kDa according to NP_071320.1). Recommended concentration: 0.1-0.3 μg/mL. an additional band at 80 kDa and a very strong 26 kDa is also observed. These bands were successfully blocked by incubation with the immunizing peptide.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Minimize freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot and store at -20 °C.
Target
TIA1
(TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))