This antibody is expected to recognize all 3 reported isoforms (as represented by NP_056084.1, NP_001009882.1, NP_001009881.1)
Aufreinigung
ZCCHC11 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ZCCHC11 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of ZCCHC11.
ELISA: Antibody detection limit dilution 1:32,000. Immunohistochemistry: In paraffin embedded tissues recommended concentration, 2-6 μg/mL. Western Blot: Approximately 250-300 kDa and 30-35 kDa band seen in Raji lysate. Recommended for use at 1-3 μg/mL.
Please note that the band we consistently detect in Western Blots differs from the predicted size of 181 kDa . However, it has several consensus glycosylation sites. Glycosylations are known to affect migration of proteins on a SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press].
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.