Resistin-like beta antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Resistin-like beta antibody was raised against a 14 amino acid synthetic peptide near the internal region of Resistin-like beta.
ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approximately 35 kDa band in human Ileum and duodenum lysates after 0.1 μg/mL antibody staining.7 kDa according to NP_115968.1. The 35 kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human colon shows textured staning of cytoplasm in enterocytes. Recommended concentration, 2-4 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.