Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

Ubiquitin Antikörper (Atto 488)

Ubiquitin Reaktivität: Rind (Kuh) WB, IHC, IF, ICC, IP Wirt: Kaninchen Polyclonal Atto 488
Produktnummer ABIN2481446
  • Target Alle Ubiquitin Antikörper anzeigen
    Ubiquitin
    Reaktivität
    • 148
    • 72
    • 71
    • 67
    • 18
    • 18
    • 16
    • 15
    • 14
    • 14
    • 14
    • 14
    • 13
    • 12
    • 11
    • 10
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Rind (Kuh)
    Wirt
    • 105
    • 75
    • 3
    • 2
    • 1
    • 1
    Kaninchen
    Klonalität
    • 100
    • 87
    Polyklonal
    Konjugat
    • 81
    • 14
    • 13
    • 11
    • 11
    • 10
    • 7
    • 7
    • 7
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Ubiquitin Antikörper ist konjugiert mit Atto 488
    Applikation
    • 168
    • 96
    • 87
    • 60
    • 55
    • 42
    • 29
    • 22
    • 21
    • 14
    • 13
    • 10
    • 4
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)
    Spezifität
    Detects ~10 kDa. It also recognizes ubiquinated proteins.
    Kreuzreaktivität
    Huhn, Rind (Kuh), Hund, Drosophila melanogaster, Fisch, Meerschweinchen, Hamster, Human, Affe, Maus, Schwein, Kaninchen, Regenbogenforelle, Ratte, Saccharomyces cerevisiae, Salmon, Schaf, Xenopus laevis
    Aufreinigung
    Peptide Affinity Purified
    Immunogen
    Native bovine Ubiquitin, conjugated to KLH
    Top Product
    Discover our top product Ubiquitin Primärantikörper
  • Applikationshinweise
    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Kommentare

    A 1:1000 dilution of ABIN2481446 was sufficient for detection of free ubiquitin in 15 μg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Conjugated antibodies should be stored at 4°C
  • Target
    Ubiquitin
    Andere Bezeichnung
    Ubiquitin (Ubiquitin Produkte)
    Synonyme
    CG11624 antikoerper, DmUb antikoerper, DmUbi-p63E antikoerper, Dmel\\CG11624 antikoerper, Ub antikoerper, Ubi-P63e antikoerper, Ubi-p antikoerper, Ubi63E antikoerper, Ubi63p antikoerper, Ubip antikoerper, Ubiq antikoerper, Ubp antikoerper, ubi antikoerper, ubi-p63E antikoerper, ubi63E antikoerper, CG18282 antikoerper, CG32744 antikoerper, CR32744 antikoerper, DmUbi-p5E antikoerper, Dmel\\CG32744 antikoerper, ubiquitin antikoerper, si:dz180g5.5 antikoerper, si:busm1-180g5.5 antikoerper, LOC100194618 antikoerper, UBIQ antikoerper, Ubiquitin antikoerper, LOC100282068 antikoerper, Ubiquitin-63E antikoerper, Ubiquitin-5E antikoerper, transmembrane and ubiquitin-like domain containing 2 antikoerper, polyubiquitin antikoerper, Putative ubiquitin family protein antikoerper, ubiquitin antikoerper, ubiquitin B antikoerper, UBiQuitin antikoerper, Ubiquitin antikoerper, Ubi-p63E antikoerper, Ubi-p5E antikoerper, tmub2 antikoerper, LOC100194618 antikoerper, pco110026 antikoerper, LOC100135798 antikoerper, UBB antikoerper, ubq-1 antikoerper, LOC100190766 antikoerper
    Hintergrund
    Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96 % sequence identity (1). The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome (2). Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate(3, 4). Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated (5). Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκBα and p27 (6).
    Gen-ID
    281370
    NCBI Accession
    NP_776558
    UniProt
    P0CG53
    Pathways
    Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
Sie sind hier:
Kundenservice