CD74 Antikörper (Biotin)

Produktnummer ABIN2481708

Produktdetails anti-CD74 Antikörper (Biotin)

Target: CD74 (HLA-DR-gamma (CD74))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD74 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Spezifität: Detects ~33-35 kDa protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74.

Kreuzreaktivität: Human, Maus

Aufreinigung: Protein G Purified

Immunogen: Human CD74 invariant chain synthetic peptide

Klon: PIN-1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• IHC (1:100)
• ICC/IF (1:50)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN2481708 was sufficient for detection of CD74 in 20 μg of PALA cell lysates by colorimetric immunolot analysis using goat anti-mouse IgG: AP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu CD74

Target: CD74 (HLA-DR-gamma (CD74))

Andere Bezeichnung: CD74 (CD74 Produkte)

Synonyme: DHLAG antikoerper, HLADG antikoerper, II antikoerper, Ia-GAMMA antikoerper, CLIP antikoerper, Ii antikoerper, INVG34 antikoerper, Iclp-1 antikoerper, iclp1 antikoerper, wu:fb11f09 antikoerper, wu:fb12a01 antikoerper, wu:fb13d06 antikoerper, wu:fk94e07 antikoerper, Iclp-2 antikoerper, cd74 antikoerper, iclp2 antikoerper, zgc:123161 antikoerper, CD74 molecule antikoerper, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antikoerper, CD74 molecule, major histocompatibility complex, class II invariant chain a antikoerper, CD74 molecule, major histocompatibility complex, class II invariant chain b antikoerper, CD74 antikoerper, Cd74 antikoerper, cd74a antikoerper, cd74b antikoerper

Hintergrund: CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC), however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).

Gen-ID: 972

NCBI Accession: NP_001020329

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints